ADP-Glo Kinase Assay + p38 gamma Kinase Enzyme System
Recombinant full-length human p38 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 gamma is a member of the p38 MAPK family, which is activated in response to stress. p38 gamma gene was mapped to 22q13.3 and functions as a signal transducer during differentiation of myoblasts to myotubes. Enforced localization of p38 gamma in the nucleus or cytoplasm markedly attenuates the ability of the kinase to induce cell cycle arrest in fibroblasts. p38 gamma increases basal glucose uptake and decreases DNP- and contraction-stimulated glucose uptake, partially by affecting levels of glucose transporter expression in skeletal muscle. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: p38 gamma, 10ug (Human, recombinant full-length). MW: ~71kDa. Substrate: p38 peptide. Other: Reaction Buffer, DTT. p38 gamma NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6300/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.