ADP-Glo Kinase Assay + PAK3 Kinase Enzyme System
Recombinant full-length mouse PAK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PAK3 is a member of the family of serine/threonine p21-activating kinases that serve as targets for the small GTP-binding proteins Cdc42 and RAC (1). The PAK family of proteins has been implicated in a wide range of biological activities and its critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK3 seems to be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in the PAK3 gene has been linked to nonsyndromic X-linked mental retardation (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: PAK3, 10ug (Mouse, recombinant full-length). MW: ~89kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. PAK3 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/18481/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.