ADP-Glo Kinase Assay + PKC delta Kinase Enzyme System
Recombinant full-length human PKC delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79kDa protein kinase that shows strict dependence on the presence of phospholipids but shows no activation by Ca2+. Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicated that PKC delta is widely distributed in almost all tissues and is a major isoform of PKC expressed in hemopoietic cells. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: PKC delta, 10ug (Human, recombinant full-length). MW: ~104kDa. Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109-121). Other: Reaction Buffer, DTT, Lipid solution. PKC delta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5580/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.