ADP-Glo Kinase Assay + PKC mu Kinase Enzyme System
Recombinant full-length human PKC mu was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C mu is a member of the protein kinase C (PKC) family that differs from the other PKC isoenzymes in structural and enzymatic properties. PKC mu is ubiquitous in nature with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKC mu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKC mu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: PKC mu, 10ug (Human, recombinant full-length). MW: ~131kDa. Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acids 109-121). Other: Reaction Buffer, DTT. PKC mu NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5587/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.