ADP-Glo Kinase Assay + RON Kinase Enzyme System
Recombinant human RON (amino acids 983-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RON is a macrophage stimulating 1 receptor c-met-related tyrosine kinase. RON receptor tyrosine kinase interacts with HYAL2 receptor protein, rendering it functionally inactive. HYAL2 is a candidate tumor-suppressor glycosylphosphatidylinositol-anchored cell-surface protein that serves as an entry receptor for jaagsiekte sheep retrovirus, a virus that causes contagious lung cancer in sheep that is morphologically similar to human bronchioloalveolar carcinoma. It was shown that RON liberated from the association with HYAL2 becomes functionally active and activates the Akt and mitogen-activated protein kinase pathways. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: RON, 10ug (Human, recombinant; amino acids 983-end). MW: ~71kDa. Substrate: Axltide (KKSRGDYMTMQIG); derived from the mouse Insulin receptor substrate 1 (amino acid 979-989). Other: Reaction Buffer, DTT. RON NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4486/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.