ADP-Glo Kinase Assay + RSK2 Kinase Enzyme System
Recombinant full-length human RSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK2 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine-threonine kinases. RSK2 has been shown to mediate growth factor signaling via RAS and MAPK, leading to the induction of CREB serine-133 phosphorylation and activation of gene expression. Mutations in RSK2 have been shown to be responsible for Coffin-Lowry syndrome (CLS), which is an X-linked disorder characterized by severe psychomotor retardation, facial and digital dysmorphisms and progressive skeletal deformations. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: RSK2, 10ug (Human, recombinant full-length). MW: ~112kDa. Substrate: RSK Substrate (KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acid 230-239). Other: Reaction Buffer, DTT. RSK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6197/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.