ADP-Glo Kinase Assay + SGK1 Kinase Enzyme System
Recombinant human SGK1 (amino acids 60-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase. SGK1 mRNA is expressed in all tissues, and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: SGK1, 10ug (Human, recombinant; amino acids 60-end). MW: ~73kDa. Substrate: Akt (PKB) substrate (CKRPRAASFAE). Other: Reaction Buffer, DTT. SGK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/6446/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.