ADP-Glo Kinase Assay + TAOK1 Kinase Enzyme System
Recombinant human TAOK1 (1-314) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs) (1). The activation of and binding to MEK3 by TAOK1 implicates TAOK1 in the regulation of the p38-containing stress-responsive MAP kinase pathway. A microtubule affinity-regulating kinase kinase, TAOK1 (also known as MARKK) is an important regulator of mitotic progression, required for both chromosome congression and checkpoint-induced anaphase delay (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: TAOK1, 10ug (Human, recombinant; amino acids 1-314). MW: ~63kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. TAOK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/57551/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.