ADP-Glo Max Assay
The ADP-Glo Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction. The assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) when higher ATP concentration is required (up to 5mM). The ADP-Glo Max Assay produces a strong signal that positively correlates with enzyme activity and can be adapted to a multitude of plate formats. The assay is performed in two steps: first, after the completion of the ADP-producing reaction, an equal volume of ADP-Glo Reagent is added to terminate the reaction and deplete the remaining ATP. Second, the ADP-Glo Max Detection Reagent is added to simultaneously convert ADP to ATP, and the latter is converted to light in a coupled reaction with luciferase/luciferin. The ADP-Glo Max Assay has a high dynamic range and produces a strong signal at low ATP to ADP conversion, making it well suited for screening low-activity ATPases such as drug membrane transporters and heat shock proteins. The assay produces minimal false hits and Z' values of greater than 0.7. Several Kinase Enzyme Systems are available. Visit www.promega.com/kinase/ to see the collection.