AKT2 Kinase Enzyme System

Recombinant full-length human AKT2 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. AKT2 or Protein Kinase B beta (PKB beta) is a serine/threonine kinase that is a member of the AKT family. AKT2 like the other AKT members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis and cell motility. The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of AKT2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: AKT2, 10ug (Human, recombinant full-length). MW: ~85kDa. Substrate: Modified AKT Substrate peptide (modified CKRPRAASFAE); based on the N-terminus of GSK3. Other: Reaction Buffer, DTT. AKT2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/208/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.