AP2 Consensus Oligonucleotide - 175pmol
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These oligonucleotides contain consensus DNA-binding sites for individual transcription factors and have 5' OH blunt ends, allowing labeling to high specific activity with T4 PNK.
The electrophoretic mobility shift assay (EMSA, gel shift, gel retardation) is a relatively simple and sensitive method to investigate protein:DNA interactions. These oligonucleotides contain consensus DNA-binding sites for individual sequence-specific transcription factors. The double-stranded oligonucleotides are designed with 5' OH blunt ends, making them easily labeled to high specific activity with T4 polynucleotide kinase.