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NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F', NM522 should be grown on minimal (M-9) medium. Genotype: supE, thi, Delta(lac-proAB), hsd5 (r-, m-), [F', proAB, lacI(q)ZDeltaM15].
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