This BSA (bovine serum albumin) has been tested and qualified for optimum performance in immunoblotting applications with alkaline phosphatase antibody conjugates. It is shown to be alkaline phosphatase-free. Tween 20 is a nonionic detergent used as a buffer component for immunoscreening in the ProtoBlot Systems. In addition to blocking agents such as BSA, which saturate excess sites of antibody binding on membranes, this detergent acts in solution to dissociate nonspecific interactions with an antibody probe.
