ChIP-IT High Sensitivity®


The ChIP-IT High Sensitivity (HS) Kit is designed to give superior performance when performing ChIP using antibodies against low abundance transcription factors, antibodies with sub-optimal binding affinities or when starting with a limited number of cells. Higher signal and lower non-specific DNA pulldown is achieved through the incorporation of specialized protein G agarose beads and optimized fixation, binding and wash buffers. In addition, washes are performed via gravity filtration, which is faster, easier and more consistent than magnetic bead capture. The high-quality ChIP DNA obtained from the ChIP-IT High Sensitivity Kit is also ideal for ChIP-Seq; ChIP-IT HS has been validated for use in this application, even when using antibodies that target low abundance transcription factors.

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