CHK1 Kinase Enzyme System

Recombinant full-length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK1 is a 56kDa serine/threonine protein kinase that was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: CHK1, 10ug (Human, recombinant full-length). MW: ~82kDa. Substrate: CHKtide (KKKVSRSGLYRSPSMPENLNRPR); derived from the human CDC25C protein isoform A (amino acid 205-225). Other: Reaction Buffer, DTT. CHK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1111/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.