CK1alpha1L Kinase Enzyme System

Recombinant full-length human CK1 alpha 1L was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1α1L or casein kinase 1, alpha 1 like is a novel member of the CKI gene family which highlights a critical role for p53 in invasiveness control. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.