DDR2 Kinase Enzyme System
Recombinant human DDR2 (467-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain (1) and is thought to be involved in the regulation of cell growth, differentiation and metabolism. DDR2 plays a role in the regulation of collagen turnover mediated by smooth muscle cells in obstructive diseases of blood vessels and the lung (2). ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: DDR2, 10ug (Human, recombinant; amino acids 467-end). MW: ~70kDa. Substrate: Axltide (CKKSRGDYMTMQIG); derived from mouse insulin receptor substrate 1 (amino acids 979-989). Other: Reaction Buffer, DTT, MnCl2. DDR2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4921/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.