DRAK2 Kinase Enzyme System

Recombinant full-length human DRAK2 (STK17B) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DRAK2 is a member of the serine/threonine kinase family and is related to death-associated protein kinase that triggers apoptosis. DRAK2 is selectively important for T-cell survival and inhibition of DRAK2 has therapeutic potential for autoimmune disease. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.