FluoroTect GreenLys in vitro Translation Labeling System
The FluoroTect Green(Lys) in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the epsilon position of the lysine with the fluorophore BODIPY-FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity. Detection of the labeled proteins is accomplished in 2-5 minutes directly in-gel by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids use. The convenience of in-gel detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.