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The GenePrint 5C Matrix Standard allows the GenePrint 24 System to be analyzed on the Applied Biosystems 3130, 3130xl, 3500 and 3500xL Genetic Analyzers. Proper generation of a spectral calibration file is critical to evaluate multicolor systems. The GenePrint 5C Matrix Standard contains matrix fragments labeled with five fluorescent dyes: Fluorescein, JOE, TMR-ET, CXR-ET and CC5. Once generated, the spectral calibration file is applied during collection of PowerPlex data to calculate and compensate for spectral overlap between different fluorescent dye colors.