Glycerol-Glo Assay

The Glycerol-Glo Assay measures glycerol using the enzymes glycerol kinase and glycerol-3-phosphate dehydrogenase in a coupled reaction scheme that links the production of NADH to the activation of a proluciferin that produces light with luciferase. The Glycerol-Glo Assay is part of a bioluminescent metabolite assay platform that offers rapid and sensitive metabolite detection in many sample types compatible with high-throughput applications. The assay can be performed in 96- and 384-well plates, and the detection reagents are added directly to samples without the need for organic extraction, making it amenable to high-throughput applications.