For superior convenience and improved yield, sensitivity and specificity, choose GoTaq G2 Hot Start Polymerase, which is bound to a proprietary antibody that blocks activity. Activity is restored during initial denaturation, but is inhibited below 70 degrees C, allowing room-temperature reaction setup. GoTaq G2 Hot Start Polymerase is supplied with 5X Green GoTaq Flexi Buffer, 5X Colorless GoTaq Flexi Buffer and 25mM MgCl2. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. In some cases, hot-start PCR may improve yields. GoTaq G2 Hot Start Polymerase exhibits 5' --> 3' exonuclease activity.