GoTaq Hot Start Polymerase
GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94-95 degrees C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70 degrees C. GoTaq Hot Start Polymerase exhibits 5' --> 3' exonuclease activity. The enzyme is supplied with a tube of 25mM MgCl2 to optimize the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq Flexi Buffer and 5X Colorless GoTaq Flexi Buffer. The buffers contain a compound that increases sample density so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.