GoTaq MDx Hot Start Polymerase
GoTaq MDx Hot Start Polymerase contains GoTaq MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94-95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields. The glycerol-free product formulation is further purified to remove glycerol, making it suitable for further manufacture processing and lyophilization. GoTaq MDx DNA Polymerase products are manufactured under cGMP. GoTaq MDx DNA Polymerase products are general purpose reagents intended for general laboratory use. They can be used as a component of molecular diagnostic assays, where applicable country laws allow, without paying royalties. The products by themselves do not provide any diagnostic result.