HaloTag Cloning Starter System

The HaloTag Cloning Starter Kit is designed to utilize the Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors. The system contains reagents required to clone and transfer gene sequences into the C- or N-terminus of a variety of HaloTag Flexi CMV Deletion Vectors. This series of vectors containing deletions of human cytomegalovirus (CMV) immediate-early enhancer/promoter were created to provide different constitutive expression levels of HaloTag 7 fusion proteins, providing greater control over the level of protein expression. This allows expression of enough fusion protein to image or capture but not so much to disturb normal function of the protein partner fused to the HaloTag 7 protein.