The HaloTag Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications. The HaloTag Succinimidyl Ester (04) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chloride separated by three ethylene glycol repeats (04). The HaloTag Succinimidyl Ester (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Succinimidyl Ester (O2) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkylchloride separated by an ethylene glycol repeat (O2). The HaloTag Succinimidyl Ester (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an amine, forming stable amide bond linkages. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Amine (04) Ligand contains a reactive amine group connected to an alkyl chloride, separated by an ethylene glycol repeat (04). The HaloTag Amine (04) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides, and TFP esters. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Amine (O2) Ligand contains a reactive amine group connected to an alkylchloride, separated by an ethylene glycol repeat (O2). The HaloTag Amine (O2) Ligand can be successfully conjugated to any reporter group, protein, or nucleic acid derivative containing an activated carboxylic acid, sulfonyl halide or isocyanate. Examples of activated carboxylic acids are succinimidyl esters, STP esters, acid halides and TFP esters. The ligand with functional group can then be used with the HaloTag protein for any application of interest. The HaloTag Iodoacetamide (04) Ligand contains a reactive iodoacetamide group connected an alkyl chloride separated by an ethylene glycol repeat (04). The HaloTag Iodoacetamide (04) Ligand has been designed to rapidly react with sulfhydryl-containing molecules, whether small organic compounds, peptides, or proteins. The ligand with functional group can then be used with the HaloTag protein for any application of interest.
