HaloTag-VHL Fusion Vector

Regulation of overall cellular protein homeostasis is critical for maintaining cell health and often found to be altered by cellular treatments or disease states. The majority of proteins and their abundance is regulated via the ubiquitin proteasome system (UPS), which uses ubiquitin conjugation to signal proteins that should be trafficked to the proteasome for degradation. Targeting proteins for degradation via the UPS with small-molecule degraders such as PROTACs or molecular glues is also emerging as a new modality for small-molecule drug development. The HaloTag fusion vectors are designed to be used as the fluorescent acceptor in NanoBRET assays that monitor interactions of a specific target protein along the UPS pathway. NanoBRET technology is a proximity-based method dependent upon energy transfer from a luminescent donor target protein to a fluorescent acceptor. In these assays the target protein fused to NanoLuc luciferase or complemented NanoBiT luciferase serves as the bioluminescent BRET energy donor. The HaloTag fusions are labeled with the HaloTag NanoBRET 618 fluorescent ligand and serve as the energy acceptor. Interactions will result in energy transfer between the donor and acceptor, resulting in increased NanoBRET signal. HaloTag-CRBN Fusion Vector contains the coding region for an N-terminal HaloTag fusion to the E3 ligase component Cereblon (CRBN). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET assay format to measure the ternary complex formation between a target protein, a degradation compound and CRBN in live cells. For more information see NanoBRET Ternary Complex Formation Starter Kits product page. The HaloTag-VHL Fusion Vector contains the coding region for an N-terminal HaloTag fusion to the von Hippel-Lindau tumor suppressor E3 ligase (VHL). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET assay format to measure the ternary complex formation between a target protein, a degradation compound and VHL in live cells. For more information see NanoBRET Ternary Complex Formation Starter Kits product page. The HaloTag-Ubiquitin Fusion Vector contains the coding region for an N-terminal HaloTag fusion to the ubiquitin protein. The vector is designed for transient or stable expression in mammalian cells and use in a NanoBRET assay format to measure ubiquitination of a target protein. For more information see the HaloTag Ubiquitination Assay Starter System product page. The HaloTag-PSMD3 Fusion Vector contains the coding region for an N-terminal HaloTag fusion to the 26S proteasome regulatory subunit 3 (PSMD3). The vector is designed for transient or stable fusion protein expression in mammalian cells and use in a NanoBRET assay format to measure trafficking of a target protein to the proteasome in live cells. For more information see Proteasome Recruitment Starter Systems product page.