HeLa nuclear extract (TNF-α stimulated)
Active Motif's HeLa nuclear extract (TNF-a stimulated) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line. The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells. Stimulation of HeLa cells with the tumor necrosis factor-alpha (TNF-a), a member of a group of cytokines that stimulate the acute phase reaction of the immune response, leads to programmed cell death, or apoptosis, and induction of activation of the NFkB signal transduction pathway. The transcription factor NFkB is widely studied due to its implication in the regulation of genes that control inflammation, cell proliferation and cell survival.