Hep G2 nuclear extract (IL-6 stimulated, 10 ng/ml)
Active Motif's Hep G2 nuclear extract (IL-6 stimulated, 10 ng/ml) was prepared from cell cultures of the human hepatocellular liver carcinoma Hep G2 cell line. These cells are epithelial in morphology and can be induced to form polarized epithelium under appropriate culture conditions. Hep G2 cells are most commonly used for studies related to hepatocyte function. Because of their ability to differentiate into polarized epithelium, these cells are often used to investigate intracellular protein trafficking, specifically in relation to human liver diseases. Hep G2 is the most commonly used cell line for studying the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are used to study liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology. In response to injury, disease or infection, interleukin-6 (IL-6) is a cytokine that is rapidly and transiently expressed as a host defense mechanism. IL-6 has various biological activities, most of which are associated with acute phase immune and inflammatory responses. In the liver, IL-6 is major regulator of acute phase protein synthesis in response to inflammation. Studies have demonstrated that constitutive IL-6 has deleterious effects on the liver and may be involved in the onset of liver disease, pathogenesis and cancer.