HL-60 nuclear extract
Active Motif's HL-60 nuclear extract was prepared from a cell culture of the human promyelocytic leukemia HL-60 cell line. The HL-60 cell line was originally derived from tumor tissue obtained from a 36 year old female patient with acute promyelocytic leukemia. HL-60 cells are primarily neutrophilic myelocyte precursors in morphology and can be induced to differentiate into granulocytic, monocytic, macrophage-like and eosinophil phenotypes depending on the compound treatment. These cells require insulin and transferrin for growth. In research, HL-60 cells are most commonly used to study myeloid differentiation, hematopoiesis, and acute promyelocytic leukemia. Historically, this cell line was used as a model system to study the effect of DNA topoisomerase IIa and IIb on differentiation and apoptosis of cells.