IKK alpha Kinase Enzyme System - MyBio Ireland - Promega

IKK alpha Kinase Enzyme System


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Full-length recombinant human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKK alpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein, which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex (1). IKK alpha is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKK alpha is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFN alpha production. IKK alpha is also involved in the epidermis, where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: IKK alpha, 10ug (Human, recombinant full-length). MW: ~114kDa. Substrate: IKKtide (KKKKERLLDDRHDSG-LDSMKDEE); derived from human IkBA (amino acids 21-41). Other: Reaction Buffer, DTT. IKK alpha NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1147/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.

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