IKK beta Kinase Enzyme System
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKKβ is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein, which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. The activity of IKKβ is stimulated by TNF and IL1 and IKKβ forms a heterodimer that interacts with NIK. Overexpression of catalytically inactive IKKβ blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKβ activity in vitro and in vivo by binding to IKKβ to reduce ATP binding. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Kinase Enzyme System contains: Kinase: IKKβ, 10microg (Human, recombinant full-length). MW: ~105kDa. Substrate: IKKtide (KKKKERLLDDRHDSG-LDSMKDEE); derived from human IkBA (amino acids 21-41). Other: Reaction Buffer, DTT. IKKβ NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/3551/. Visit www.promega.com/kinase/ to see all Kinase Enzyme Systems.