Klenow Fragment, Exonuclease Minus
DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5'-->3' and the 3'-->5' exonuclease activities present in intact E. coli DNA Polymerase I. It is used for random primer labeling and in strand displacement amplification. Klenow Fragment, Exonuclease Minus, will leave a single-base 3' overhang on a significant proportion of DNA fragments during fill-in of 5'-overhangs. Therefore, this enzyme is not recommended for preparation of blunt-ended fragments for ligation.