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COVID-19 Rapid Antigen and Antibody Tests - Saliva, Nasal and Nasopharyngeal
COVID-19 Rapid Antigen and Antibody Tests - Saliva, Nasal and Nasopharyngeal

KSR1 (L639F) Kinase Enzyme System

by Promega
€ 455.35
Size

Recombinant human KSR1 (L639F) (335-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KSR1 or Kinase Suppressor of Ras 1 interacts with various kinases of the Raf/MEK/extracellular signal-regulated kinase pathway to enhance its activation. KSR1 is regulated in response to a specific mode of dimerization of its kinase domain, which is termed the side-to-side dimer, whereas KSR1 also participates in forming side-to-side heterodimers with RAF and this can trigger RAF activation. KSR1 has an essential protective role in the intestinal epithelial cell during inflammation through activation of cell survival pathways. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.