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L-Rhamnose Monohydrate

by Promega
€ 47.78
Size

L-Rhamnose Monohydrate is used to induce protein expression in vectors cloned into Single Step (KRX) Competent Cells. Single Step (KRX) is an E. coli K strain that contains a chromosomal copy of the T7 RNA polymerase driven by a rhamnose promoter (rhaBAD) to provide dramatic control of the proteins expressed via a T7 promoter. Pre-induced expression protein levels are significantly lower than those of BL21(DE3)-derived strains. This feature facilitates cloning and expression of proteins toxic to E. coli.