LightSwitch™ 3´UTR GoClone®
The LightSwitch 3'UTR GoClone Collection contains over 12,000 3'UTR sequences that have been cloned from the human genome into the LightSwitch Luciferase Reporter vector, simplifying the tasks of performing miRNA target validation and assessing the functional impact of miRNA-3'UTR interactions. The clones have been sequence-verified and are supplied transfection-ready, so you can begin your reporter assays immediately, without the need to clone or prepare DNA. Simply visit the Active Motif website to find your clones of interest using our online search tool, then transfect into your cell line of interest and assay using our optimized reagents and positive and negative controls. Also available are collections of miRNA mimics and inhibitors, as well as clones that contain synthetic miRNA target sequences that can often provide more sensitive screening assays than the endogenous 3'UTR sequences. Custom services are also offered.