Luciferase 1000 Assay System
The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least 8 orders of magnitude of enzyme concentration, and the patented technology incorporated into this assay system allows measurement of less than 10(-20) moles of luciferase under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over chloramphenicol acetyltransferase (CAT) assays. The Luciferase Assay Reagent generates light that is nearly constant for at least 1 minute and so is compatible with measuring firefly luciferase in a single-tube luminometer or in a multiwell plate luminometer with an auto-injector. The Luciferase Assay System is a nonhomogeneous assay system; the cells containing the luciferase must be lysed before reagent addition. Glo Lysis Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) can be used with the Luciferase Assay System for reporter quantitation in mammalian cells. The Luciferase Assay System also can be used for quantitation in plant and bacterial cells, but only Cell Culture Lysis Reagent is suitable for these applications. Reporter Lysis Buffer enables firefly luciferase, CAT and beta-galactosidase assays to be performed from the same cell extract. In some kits the lysis buffer is included, and in others it must be purchased separately.