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Lumit™ HMGB1 (Human/Mouse) Immunoassay

by Promega
€ 531.25
Size

Product Description and Background: The Lumit™ HMGB1 (Human/Mouse) Immunoassay is a homogeneous, bioluminescent assay for detecting high-mobility group box 1 (HMGB1) released from cells without the need for sample transfers or wash steps.

HMGB1 is a highly abundant and evolutionary conserved protein that has many important biological activities in intracellular and extracellular environments. HMGB1 typically resides with DNA and histones inside the nucleus to regulate transcription and help order and shape chromatin structure. When HMGB1 is translocated to the cytoplasm, it can help mediate autophagy. During a process called Immunogenic Cell Death (ICD), tumor or oncolytic virus-infected cells release HMGB1 via either active or passive mechanisms in response to ICD-inducing treatments. Once outside the dying tumor cell, HMGB1 can serve as a Damage-Associated Molecular Pattern (DAMP) molecule, which can help stimulate recruitment of dendritic cells to the tumor bed.

Immunogenic Cell Death: Typically, when cells die by apoptosis, a process that occurs millions of times per day in the human body, there is no immune response to the cell death, and apoptosis is deemed “silent” to the immune system. Immunogenic Cell Death (ICD) is a specialized form of apoptosis (programmed cell death) that elicits an immune response. ICD is characterized by the ability of dying cells to elicit a robust adaptive immune response against tumor cells or infected cells. Some cancer treatments, including specific chemotherapeutic drugs, radiation, oncolytic viruses, and others, induce ICD, and lead to better long-term outcomes for the patient due to the activation of the immune system. This is also referred to as “turning a cold tumor hot,” and combination therapeutics that provoke an immunogenic response are of significant interest in the immuno-oncology drug development area. Therefore, there is a need to find treatments that induce ICD.

Assay Principle: Primary monoclonal antibodies to HMGB1 are labeled with SmBiT and LgBiT. In the presence of the HMGB1, SmBiT and LgBiT are brought into proximity, forming the NanoBiT® enzyme. When Lumit™ Detection Reagent B is added, a bright luminescent signal is generated in proportion to the amount of HMGB1 present.


Value Proposition: Lumit assays are a fast, easy and scalable alternative to ELISAs for analyte detection.

Positioning: Bench Scientist User Persona, Lumit versus ELISA

For scientists detecting HMGB1 from cell-culture samples who need cost-effective, reproducible and robust assays,

Lumit HMGB1 Immunoassay is a no-wash, ready-to-use assay that saves precious time, labor and resources.

Unlike competing ELISA methods, we streamline your workflow so you have time to take your whole lunch break.

Positioning: Group Leader Decision Maker Persona, Lumit versus PerkinElmer

For scientists detecting HMGB1 from cell-culture samples who need cost-effective, reproducible and robust assays using standard lab equipment,

Lumit HMGB1 Immunoassay is a no-wash, ready-to-use assay that saves precious time, labor and resources.

Unlike competing HTRF methods, Lumit™ assays don’t require expensive capital expenditures.

Market Segments and Target Customers:

  • Drug Discovery and Development: Pharma/Biotech/CROs
    Higher throughput users in drug discovery and development use large number of plates at once—will appreciate fast and easy protocol
  • Basic Research: Academic/Government, Clinical/Hospital (research labs)—will appreciate feature of ready-to-use assay (save time on assay development) and time savings

Features and Benefits:

  • Direct, in-well detection: saves time and resources (cells, reagents, plastics) in the lab
  • No wash steps required; simple add-mix-measure protocol: saves time and resources in the lab, no need to use plate washer instrument, less opportunity for error with fewer hands-on steps
  • Broad linear range: sample dilutions not required à saves time and resources in the lab
  • No specialized instrumentation required: saves money
  • Validated assays for high-value target: saves time (weeks to months) of developing an assay