Skip to content
COVID-19 Rapid Antigen and Antibody Tests - Saliva, Nasal and Nasopharyngeal
COVID-19 Rapid Antigen and Antibody Tests - Saliva, Nasal and Nasopharyngeal

Lys-C, Mass Spec Grade

by Promega
€ 418.87
Size

Endoproteinase Lys-C is a serine protease isolated from Lysobacter enzymogenes as a highly purified protease that hydrolyzes specifically at the carboxyl side of Lys. Lys-C retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. Lys-C activity is optimal in the pH range of 7.0-9.0. This protease can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.Endoproteinase Lys-N is a metalloprotease that cleaves at the amino side of lysine residues. Lys-N is a zinc metalloprotease derived from Grifola frondosa that retains proteolytic activity under protein denaturing conditions such as 8M urea, which is used to improve digestion of proteolytically resistant proteins. Charged amino-terminal peptide fragments generated by Lys-N are useful for de novo sequencing with ETD fragmentation techniques.