MagneSil Total RNA mini-Isolation System
The MagneSil Total RNA mini-Isolation System provides a high-throughput 96-well format for fast, simple preparation of intact total RNA from small amounts of cell culture (=1 x 10(5) tissue culture cells), tissue (=2mg tissue lysate in 100ul) or freshly isolated whole blood (=20ul). The protocol enables high-throughput automated purification on a variety of liquid-handling workstations. Isolation of total RNA in a 384-well format from cell culture (=1 x 10(3) cells) and freshly isolated whole blood (=5ul) also may be performed. Total RNA purification is achieved without vacuum filtration, centrifugation or precipitation. The 96-well total RNA isolation procedure takes about 30 minutes to complete using a liquid-handling workstation. Total RNA purified using the MagneSil Total RNA mini-Isolation System is suitable for a variety of molecular biology applications including endpoint RT-PCR amplification and real-time RT-PCR.