MASTL Kinase Enzyme System
Full-length recombinant human MASTL was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Microtubule associated serine/threonine kinase-like (MASTL) belongs to the MAST family, AGC Ser/Thr protein kinase group. MASTL promotes mitotic progression and cell cycle reentry after DNA damage. Loss of MASTL leads to defects in chromosome condensation, separation, and other aspects of mitotic progression, which is primarily mediated by inhibiting PP2A/B55δ. Overexpression of MASTL is correlated with cancer progression, poor patient survival, and tumor recurrence in various cancers. ADP-Glo Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.