Maxwell® CSC RNA Blood Kit

by Promega
Available SKUs: AS1410 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Maxwell® CSC RNA Blood Kit Purifies High-Quality RNA

    The Maxwell® CSC RNA Blood Kit is intended for use as an in vitro diagnostic (IVD) medical device, in combination with the Maxwell® CSC Instrument, to provide an easy method for efficient, automated purification of RNA from 2.5ml fresh human whole blood collected in EDTA tubes. The kit uses predispensed reagent cartridges to maximize simplicity and convenience and reduce variability.

    The Maxwell® CSC Instrument is preprogrammed with the purification method and can process up to 16 samples in approximately 60 minutes. The purified RNA can be used directly in a variety of amplification-based downstream applications, such as RT-PCR.

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    Reliable Consistent RNA Purity

    Figure 12703MA
    RNA Purity ratios A260/A280; A260/A230 are shown. RNA was purified in triplicate from eight samples using the Maxwell® CSC Instrument and the Maxwell® CSC RNA Blood Kit. Absorbance was measured at 230nm, 260nm and 280nm using a spectrophotometer. Average and standard deviation of triplicate samples are shown.

    RNA Performs Well in Downstream Amplification Assays

    12705MA_maxwell_csc_rna_1200px
    Purified RNA was tested for inhibition in RT-qPCR amplification. RNA was purified in eight replicates from 2.5ml of whole blood using the Maxwell® CSC Instrument with the Maxwell® CSC RNA Blood Kit. ΔCq of purified RNA from sample was compared to a no-inhibition control (NIC) and no significant difference in amplification was observed.

    Product currently only available in the United States, Canada, China and Korea.

  • Protocols

    Video Protocols

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  • Specifications

    What's in the box?

    Item Part # Size

    CSC/RSC Plungers

    AS133A 1 × 50/pk

    Maxwell® CSC RNA Blood Cartridge

    AS141A 48 × 1 each

    Elution Tubes (0.5ml)

    AS620A 1 × 50/pk

    Lysis Buffer

    MC110A 1 × 20ml

    Proteinase K (PK) Solution

    MC111A 2 × 1ml

    Nuclease-Free Water

    MC119A 1 × 25ml

    Blue Dye

    MC122A 1 × 100μl

    Solution A

    MC130A 4 × 100ml

    Solution B

    MC131A 1 × 30ml

    1-Thioglycerol

    MC132A 1 × 900μl

    DNase I (lyophilized)

    Z358A 2 × 1 vial

    Certificate of Analysis

    See all certificates for AS1410

    Use Restrictions

    For In Vitro Diagnostic Use. This product is only available in certain countries.

    Storage Conditions

    DD

    Patents and Disclaimers

    U.S. Pat. Nos. 6,027,945 and 6,368,800.

    U.S. Pat. No. 7,329,488 and other patents.

  • Resources

    Software and Firmware

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