Maxwell® RSC PureFood Pathogen Kit

by Promega
Available SKUs: AS1660 |
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources
  • Take a Closer Look and Take Control of Your Processes

    If you need to make quick decisions about potential food spoilage and contamination, the Maxwell® RSC PureFood Pathogen Kit offers a simple extraction protocol to obtain high-quality bacterial DNA from a variety of food sample types. The kit works with inhibiting sample types, and can lyse both Gram+ or Gram– bacteria, eliminating laborious sample preparation steps like enzymatic pretreatment. 

    The extracted DNA is ready for advanced downstream molecular analyses including NGS, serotyping, and identification of spoilage organisms. The high-performance Maxwell® chemistries coupled with the trusted benchtop Maxwell® RSC instrument allow you to purify bacterial DNA from food samples in as little as 40 minutes, giving you the ability to get answers more quickly.

    pathogen-overview-image

    Isolate Pathogen DNA from Many Food Sample Types

    This method has been used to isolate pathogen DNA from a number of different food samples including E. coli 0157:H7 from uncooked beef, Salmonella enterica from uncooked chicken, and Listeria monocytogenes from whole milk.
    14884ma-w
    Comparison of total DNA and E. coli 0157:H7 DNA extracted from cilantro samples spiked with the indicated amounts of  E. coli 0157:H7 bacteria. The total DNA concentration was assessed using the QuantiFluor® ONE dsDNA System.
    14885ma-w
    Comparison of total DNA and E. coli 0157:H7 DNA extracted from cilantro samples spiked with the indicated amounts of  E. coli 0157:H7 bacteria. The concentration of  E. coli 0157:H7  DNA was assessed by qPCR with specific primers.

    Advantages

    Simple, automated protocol requires minimal hands-on steps.
    Avoid organic extractions and laborious sample preprocessing steps.
    Use an optimized system to extract bacterial DNA from many food samples.
  • Item Part # Size

    Lysis Buffer

    A826D 1 × 25ml

    Elution Buffer

    A828D 1 × 20ml

    Maxwell® RSC Cartridge (RSCJ)

    AS159A 48 × 1 each

    Elution Tubes (0.5ml)

    AS620A 1 × 50/pk

    Maxwell® RSC Plunger Pack

    AS1670 1 × 48/pack

    Lysis Buffer A

    MC143A 1 × 20ml

    Use Restrictions

    Not For Medical Diagnostic Use.

    Storage Conditions

    DD

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