Maxwell® RSC PureFood Pathogen Kit

by MyBio Ireland
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Description

  • Overview
  • Protocols
  • Specifications
  • Resources

High-Quality Bacterial DNA from Food Samples

  • Isolate DNA from raw or processed food samples
  • Works well with inhibiting sample types
  • No need for labor-intensive sample processing

Size

Catalog number selected: AS1660

$ 295.00 Your price:

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Take a Closer Look and Take Control of Your Processes

If you need to make quick decisions about potential food spoilage and contamination, the Maxwell® RSC PureFood Pathogen Kit offers a simple extraction protocol to obtain high-quality bacterial DNA from a variety of food sample types. The kit works with inhibiting sample types, and can lyse both Gram+ or Gram– bacteria, eliminating laborious sample preparation steps like enzymatic pretreatment. 

The extracted DNA is ready for advanced downstream molecular analyses including NGS, serotyping, and identification of spoilage organisms. The high-performance Maxwell® chemistries coupled with the trusted benchtop Maxwell® RSC instrument allow you to purify bacterial DNA from food samples in as little as 40 minutes, giving you the ability to get answers more quickly.

pathogen-overview-image

Isolate Pathogen DNA from Many Food Sample Types

This method has been used to isolate pathogen DNA from a number of different food samples including E. coli 0157:H7 from uncooked beef, Salmonella enterica from uncooked chicken, and Listeria monocytogenes from whole milk.
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Comparison of total DNA and E. coli 0157:H7 DNA extracted from cilantro samples spiked with the indicated amounts of  E. coli 0157:H7 bacteria. The total DNA concentration was assessed using the QuantiFluor® ONE dsDNA System.
14885ma-w
Comparison of total DNA and E. coli 0157:H7 DNA extracted from cilantro samples spiked with the indicated amounts of  E. coli 0157:H7 bacteria. The concentration of  E. coli 0157:H7  DNA was assessed by qPCR with specific primers.

Advantages

Simple, automated protocol requires minimal hands-on steps.
Avoid organic extractions and laborious sample preprocessing steps.
Use an optimized system to extract bacterial DNA from many food samples.
    • Protocols Content
    • Specifications Content

Specifications

What's in the box?

Item Part # Size Available Separately

Lysis Buffer

A826D 1 × 25ml View Product

Elution Buffer

A828D 1 × 20ml View Product

Maxwell® RSC Cartridge (RSCJ)

AS159A 48 × 1 each

Elution Tubes (0.5ml)

AS620A 1 × 50/pk View Product

Maxwell® RSC Plunger Pack

AS1670 1 × 48/pack View Product

Lysis Buffer A

MC143A 1 × 20ml

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

DD
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