Using the Nano Glo HiBiT Blotting System, any HiBiT tagged proteins can be visualized on membranes following gel separation. The blotting reagent, which contains the LgBiT Protein and furimazine substrate, is added directly to the membrane, and a luminescent signal is produced only where HiBiT is present.This simple method requires as few as 5 minutes to perform in contrast to the multiple hours and many steps needed for standard antibody-based blotting protocols. Because luminescence is only produced only where HiBiT is present, background is minimal.
