With the Nano Glo HiBiT Lytic Detection System, the quantity of HiBiT tagged protein is measured directly in cell lysates with a simple add-mix-read assay. The Nano Glo HiBiT Detection Reagent is added to cells expressing HiBiT tagged proteins, lysing the cells and providing the LgBiT Protein and furimazine substrate necessary for luminescence.The high affinity interaction between LgBiT Protein and HiBiT tag reconstitutes the luminescent NanoBiT enzyme. This results in a highly quantitative assay with fewer processing steps compared to standard antibody-based detection methods. The broad linear dynamic range accurately quantifies tagged proteins regardless of expression level to measure changes in protein abundance. With a limit of detection of less than 10e^-19 moles, the Nano Glo HiBiT Lytic Detection System can quantify even low abundance proteins at endogenous levels of expression.
