Nano-Glo In-Gel Detection System
The Nano-Glo In-Gel Detection System directly detects the luminescence of NanoLuc fusion proteins in a polyacrylamide gel matrix, following protein separation by polyacrylamide gel electrophoresis (PAGE) under either native or denaturing SDS conditions (native PAGE or SDS-PAGE). Compared to Western blotting (immunoblotting), the Nano-Glo In-Gel Detection System offers superior speed, sensitivity and specificity with a simple protocol that does not require transfer to membranes, eliminating the need for blocking or antibodies. Following native PAGE, NanoLuc activity is maintained, so the gels can be placed directly into Nano-Glo In-Gel Detection Reagent and imaged. Following SDS-PAGE, the denaturing gels are first washed with isopropanol and water to remove the SDS detergent and allow protein refolding prior to adding detection reagent and imaging the gel.