The Nano-Glo Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media. The reagent is prepared by mixing Nano-Glo Luciferase Assay Substrate and Nano-Glo Luciferase Assay Buffer. The reagent contains an integral lysis buffer allowing use directly on cells expressing NanoLuc luciferase or the culture media when luciferase is secreted. Nano-Glo Luciferase Assay Reagent is a dedicated product for the detection of NanoLuc Luciferase. For more details on NanoLuc Luciferase, visit the NanoLuc Luciferase Technology page.