The NanoBRET Nano-Glo Detection Systems provide the NanoBRET Nano-Glo Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag NanoBRET 618 Ligand for the fluorescent energy acceptor. The HaloTag NanoBRET 618 Ligand is added directly to the cells during plating, and the NanoBRET Nano-Glo Substrate is added to the sample just prior to measuring donor and acceptor emission. The NanoBRET Nano-Glo Kinetic Detection System has the Vivazine substrate for use by the NanoLuc luciferase donor, giving an extended signal for live-cell kinetic assays. For more details on using the NanoBRET assay for protein:protein interaction studies visit the NanoBRET Technology for Protein Interactions page.
