NanoBRET VHL Ternary Complex Starter Kit
Targeted degradation via small molecule degraders such as PROTACs or molecular glues require forming a ternary complex that consists of target protein:degrader compound:E3 ligase component. Ternary complex formation is the first mechanistic step required for target ubiquitination and degradation via the ubiquitin proteasome pathway, representing a key step in optimizing effective degrader compounds. The NanoBRET Ternary Complex Starter Kits provide the tools to create target-specific assays for live-cell detection of complex formation with either the VHL (von Hippel-Lindau disease tumor suppressor) or CRBN (cereblon) E3 ligase components. These assays use NanoBRET technology, a proximity-based method dependent upon energy transfer from a luminescent donor target protein to a fluorescent acceptor and can be performed using either endpoint or kinetic format to monitor the rate and stability of complex formation. The ratio of the bioluminescent and fluorescent signal in the NanoBRET assay means you can measure ternary complex formation during target protein degradation and simultaneously monitor target protein levels.